{"id":736,"date":"2019-04-13T19:30:22","date_gmt":"2019-04-13T19:30:22","guid":{"rendered":"https:\/\/ccsb.scripps.edu\/agfr\/?page_id=736"},"modified":"2019-05-15T20:10:55","modified_gmt":"2019-05-15T20:10:55","slug":"boxligand","status":"publish","type":"page","link":"https:\/\/ccsb.scripps.edu\/agfr\/boxligand\/","title":{"rendered":"boxLigand"},"content":{"rendered":"<p class=\"last-modified\">This page was last updated on May 15th, 2019 at 08:10 pm<\/p><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-one-full fusion-column-first fusion-column-last\" style=\"--awb-bg-size:cover;\"><div class=\"fusion-column-wrapper fusion-flex-column-wrapper-legacy\"><div class=\"fusion-text fusion-text-1\"><h3>Use case 1: docking box define using a known ligand<\/h3>\n<p>In this scenario we have a receptor and a known ligand in the PDBQT format and we define a docking box centered on the ligand.<\/p>\n<p>We will use the cyclic dependent kinase protein 2 CDK2 (pdbid:4EK3) and one of its ligands (pdbid:4EK4). The files 4EK3_rec.pdbqt and 4EK4_lig.pdbqt are available in the <a href=\"https:\/\/ccsb.scripps.edu\/adfr\/download\/1063\/\">data file associated with this tutorial.<\/a><\/p>\n<p>run\u00a0<em><strong>ADFRsuite-1.0\/bin\/agfrgui<\/strong><\/em>\u00a0to start the Graphical User interface for these tutorials.<\/p>\n<\/div><div class=\"fusion-tabs fusion-tabs-1 clean vertical-tabs icon-position-left mobile-mode-accordion\" style=\"--awb-title-border-radius-top-left:0px;--awb-title-border-radius-top-right:0px;--awb-title-border-radius-bottom-right:0px;--awb-title-border-radius-bottom-left:0px;--awb-inactive-color:#9acae0;--awb-background-color:#ffffff;--awb-border-color:#9acae0;--awb-active-border-color:#d65169;\"><div class=\"nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li class=\"active\" role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-02143be54a1fc0adad8\" aria-selected=\"true\" id=\"fusion-tab-loadreceptor\" href=\"#tab-02143be54a1fc0adad8\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-keyboard fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Load receptor<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-a216f3748a27357ba0f\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-loadligand\" href=\"#tab-a216f3748a27357ba0f\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-keyboard fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Load ligand<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-35103d1bc00a8ae140a\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-definebox\" href=\"#tab-35103d1bc00a8ae140a\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Define box<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-36e0033f64e842611ee\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-setautositeversion\" href=\"#tab-36e0033f64e842611ee\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Set AutoSite version<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-2efd4aec64d8bbce3eb\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-bindingpockets\" href=\"#tab-2efd4aec64d8bbce3eb\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Binding pockets<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-139ac17b0c82db5d781\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-mapsparameters\" href=\"#tab-139ac17b0c82db5d781\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Maps parameters<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-b6d089c44ce9385d9ec\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-generatemaps\" href=\"#tab-b6d089c44ce9385d9ec\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-calculator fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Generate maps<\/h4><\/a><\/li><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-8b65585dfa49888edc6\" aria-selected=\"false\" tabindex=\"-1\" id=\"fusion-tab-cmdline\" href=\"#tab-8b65585dfa49888edc6\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-calculator fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>cmd line<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-content\"><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li class=\"active\" role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-02143be54a1fc0adad8\" aria-selected=\"true\" id=\"mobile-fusion-tab-loadreceptor\" href=\"#tab-02143be54a1fc0adad8\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-keyboard fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Load receptor<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix in active\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-loadreceptor\" id=\"tab-02143be54a1fc0adad8\">\n<h4><span style=\"color: #008080;\">Click\u00a0<img decoding=\"async\" class=\"wp-image-743 alignnone\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-8.50.57-AM.png\" alt=\"\" width=\"96\" height=\"40\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-8.50.57-AM-200x83.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-8.50.57-AM.png 226w\" sizes=\"(max-width: 96px) 100vw, 96px\" \/> and select 4EK3_rec.pdbqt<\/span><\/h4>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:1px;border-bottom-width:1px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p>Details: the receptor molecule is loaded and displayed as line representing atomic bonds colored by atom type with carbon atoms shown in cyan. The default docking-box covers the entire receptor with the default padding (4.0 Angstroms) added to each side.<\/p>\n<p><img decoding=\"async\" width=\"995\" height=\"770\" class=\"wp-image-508 aligncenter\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1.png\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1-200x155.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1-300x232.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1-400x310.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1-600x464.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1-768x594.png 768w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1-800x619.png 800w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-1.png 995w\" sizes=\"(max-width: 995px) 100vw, 995px\" \/><\/p>\n<p>NOTES:<\/p>\n<ul>\n<li>Amino acids located in the docking box with no flexible side-chains (<em>i.e.<\/em> glycine, alanine and proline) are displayed with dimmed down lines.<\/li>\n<li>Several buttons in the control section and the tool-bar are now enabled.<\/li>\n<li>The status bar indicates that the next step could be to detect pockets.<\/li>\n<\/ul>\n<p>The 3D scene can be rotated, translated and scaled using the 3 mouse buttons:<\/p>\n<div class=\"table-1\">\n<table style=\"height: 142px;\" width=\"251\">\n<tbody>\n<tr>\n<td style=\"text-align: left;\">Mouse button<\/td>\n<td style=\"text-align: left;\">Action<\/td>\n<\/tr>\n<tr>\n<td>Left<\/td>\n<td>Rotate<\/td>\n<\/tr>\n<tr>\n<td>Middle<\/td>\n<td>Translate<\/td>\n<\/tr>\n<tr>\n<td>Right<\/td>\n<td>Scale<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>Depth-cueing can be turned on and off by pressing the keyboard key \u2018d\u2019 while the mouse pointer is in the 3D view.<\/p>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-a216f3748a27357ba0f\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-loadligand\" href=\"#tab-a216f3748a27357ba0f\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-keyboard fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Load ligand<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-loadligand\" id=\"tab-a216f3748a27357ba0f\">\n<h4><span style=\"color: #008080;\">Click on the <img decoding=\"async\" class=\"wp-image-509\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-2.png\" width=\"80\" height=\"46\" \/> button to load 4EK4_lig.pdbqt<\/span><\/h4>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:1px;border-bottom-width:1px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p>Details: the ligand molecule appears in the licorice representation with carbon atoms displayed in yellow.<\/p>\n<p><img decoding=\"async\" width=\"894\" height=\"692\" class=\"wp-image-510 aligncenter\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3.png\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3-200x155.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3-300x232.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3-400x310.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3-600x464.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3-768x594.png 768w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3-800x619.png 800w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-3.png 894w\" sizes=\"(max-width: 894px) 100vw, 894px\" \/><\/p>\n<p>NOTE:<\/p>\n<ul>\n<li>More buttons are enabled by this operation both in the control panel and in the tool-bar. In particular the button allowing to position and size the box using the ligand\u00a0<img decoding=\"async\" class=\"size-full wp-image-655 alignnone\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-57.png\" alt=\"\" width=\"47\" height=\"47\" \/>.<\/li>\n<\/ul>\n<h2><\/h2>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-35103d1bc00a8ae140a\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-definebox\" href=\"#tab-35103d1bc00a8ae140a\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Define box<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-definebox\" id=\"tab-35103d1bc00a8ae140a\">\n<h4><span style=\"color: #008080;\">Click\u00a0\u00a0<img decoding=\"async\" width=\"47\" height=\"47\" class=\"wp-image-512\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-5.png\" \/>\u00a0to make the docking box cover the ligand then click on <img decoding=\"async\" width=\"42\" height=\"40\" class=\"wp-image-513\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-6.png\" \/> to focus the 3D scene on the box.<\/span><\/h4>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:1px;border-bottom-width:1px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p>Details: the docking box is now centered on the ligand and scaled to have a 4.0 angstroms padding from the ligand atoms.<\/p>\n<p>The padding can be adjusted using the spin box in the \u201cdocking box\u201d section.<\/p>\n<p><img decoding=\"async\" class=\"alignleft size-full wp-image-511\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4.png\" alt=\"\" width=\"968\" height=\"751\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4-200x155.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4-300x233.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4-400x310.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4-600x465.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4-768x596.png 768w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4-800x621.png 800w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-4.png 968w\" sizes=\"(max-width: 968px) 100vw, 968px\" \/><\/p>\n<p>NOTES:<\/p>\n<ul>\n<li>The amino acids outside the docking , as well as the amino acids within the docking box with no flexible side-chains (i.e. glycine, alanine and proline) are displayed dimmed down. This facilitates the visual identification of the amino acids in the docking box that can potentially be made flexible during the docking calculation.<\/li>\n<li>Click the <img decoding=\"async\" class=\"wp-image-514\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-7.png\" width=\"32\" height=\"30\" \/> button to display the \u201cbox parameters\u201d user interface. This interface provides widgets to modify the box center coordinates, and box size along the 3 dimensions.<img decoding=\"async\" class=\"wp-image-515 aligncenter\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-8.png\" width=\"367\" height=\"218\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-8-200x119.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-8-300x178.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-8-400x238.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/word-image-8.png 459w\" sizes=\"(max-width: 367px) 100vw, 367px\" \/> NOTE: the mouse scroll wheel can be used to alter values while the mouse pointer is over the widget displaying numerical values.<\/li>\n<\/ul>\n<p>The spacing widget sets the distance between the grid points at which affinity values are calculated.<\/p>\n<p>The smoothing widget allows the specification of the smoothing factor used for calculating affinity values. These values are initialized with AutoGrid\u2019s default values.<\/p>\n<p>The <em>cmd<\/em> typein widget allows the command-line specification of the docking box. For instance: center 10 11 12.3 will center the docking-box on (10.0, 11.0, 12.3). Multiple commands can be entered on a single line separated by a semicolon character \u2018;\u2019 (e.g. \u201ccenter 12.34 34.12 56.56; size 26 26 26&#8243;) .<\/p>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-36e0033f64e842611ee\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-setautositeversion\" href=\"#tab-36e0033f64e842611ee\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Set AutoSite version<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-setautositeversion\" id=\"tab-36e0033f64e842611ee\">\n<h4><span style=\"color: #008080;\">Click on <img decoding=\"async\" class=\" wp-image-517 alignnone\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/a-close-up-of-a-mans-face-description-automatical.png\" alt=\"A close up of a mans face Description automatically generated\" width=\"68\" height=\"29\" \/>\u00a0and select 1.0<br \/>\n<\/span><\/h4>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:1px;border-bottom-width:1px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p>Details: versions 1.0 and 1.1 of\u00a0<a href=\"http:\/\/adfr.scripps.edu\/autosite\"><em>AutoSite<\/em><\/a>\u00a0are available. Version 1.1 produces better pocket descriptions, especially for larger ligands, but is somewhat slower. Here we use version 1.0 as it displays more grid points which work better for illustrative purposes.<\/p>\n<p><img decoding=\"async\" class=\" wp-image-613 aligncenter\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/a-screenshot-of-a-cell-phone-description-automati-1.png\" alt=\"A screenshot of a cell phone Description automatically generated\" width=\"339\" height=\"182\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/a-screenshot-of-a-cell-phone-description-automati-1-200x107.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/a-screenshot-of-a-cell-phone-description-automati-1-300x161.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/a-screenshot-of-a-cell-phone-description-automati-1-400x214.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/a-screenshot-of-a-cell-phone-description-automati-1-600x322.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/a-screenshot-of-a-cell-phone-description-automati-1.png 612w\" sizes=\"(max-width: 339px) 100vw, 339px\" \/><\/p>\n<p>NOTES:<\/p>\n<ul>\n<li>Version 1.1 of AutoSite performs post processing steps on the fills identified by version 1.0. First it inflates the fills to achieve better coverage of known ligands, and second it shrinks the inflated fill to retain 1\/5 of the fill points to generate better points to be used during docking to position the ligand in the box.<\/li>\n<li>Version 1.1 can use a custom scoring function for ranking pockets when the ligand is a peptide.<\/li>\n<li>Version 1.1 uses a ligand size (i.e. volume expressed in grid points) to rank pockets. The default values of 500 selects good pockets for both small molecules and peptides.<\/li>\n<\/ul>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-2efd4aec64d8bbce3eb\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-bindingpockets\" href=\"#tab-2efd4aec64d8bbce3eb\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Binding pockets<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-bindingpockets\" id=\"tab-2efd4aec64d8bbce3eb\">\n<h4><span style=\"color: #008080;\">Click on <img decoding=\"async\" class=\" wp-image-755 alignnone\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.14-AM.png\" alt=\"\" width=\"254\" height=\"24\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.14-AM-200x19.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.14-AM-300x28.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.14-AM-400x38.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.14-AM-540x52.png 540w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.14-AM.png 550w\" sizes=\"(max-width: 254px) 100vw, 254px\" \/><br \/>\n<\/span><\/h4>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:1px;border-bottom-width:1px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p>Details: ligand binding pockets are computed using <a href=\"http:\/\/adfr.scripps.edu\/autosite\"><em>AutoSite<\/em><\/a>\u00a0by identifying and cluster high affinity points. Each cluster of points is called a &#8220;fill&#8221; and listed in the table widget. The pocket with the largest <em>AutoSite<\/em> score is selected by defaults and displayed as a set of green dots. The fill points of all selected fills are displayed in the 3D viewer and will be written into the target files.<\/p>\n<p><img decoding=\"async\" class=\"alignleft size-full wp-image-756\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM.png\" alt=\"\" width=\"2134\" height=\"1652\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-200x155.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-300x232.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-400x310.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-600x464.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-768x595.png 768w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-800x619.png 800w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-1024x793.png 1024w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM-1200x929.png 1200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.06.33-AM.png 2134w\" sizes=\"(max-width: 2134px) 100vw, 2134px\" \/><\/p>\n<p>NOTES:<\/p>\n<ul>\n<li><em>AGFR<\/em> supports version 1.0 and 1.1 of the <em>AutoSite<\/em> algorithm. The latter is slower but creates better pocket descriptors, in particular for larger ligands such as peptides.<\/li>\n<li>Binding pockets are defined as subsets of high affinity grid points calculated on a 1 Angstrom resolution grid for the carbon, oxygen, and hydrogen maps (C, NA, HD). These points are clustered. Each cluster is called a fill and is assigned a rank. Usually, high ranking clusters are likely to be ligand binding pockets.<\/li>\n<li>Additional fills can be selected. The fill points of all selected fills are displayed as dots and will be saved in the target file. <em>ADFR<\/em>\u00a0will use these points during docking for positioning the ligand in the docking box.<\/li>\n<li>The button labeled \u201cgenerate target file\u2026\u201d is now enabled and all required status lights are green indicating that we defined a valid docking box and a valid pocket definition (<em>i.e.<\/em> a set of fill points that overlap with the docking box).<\/li>\n<\/ul>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-139ac17b0c82db5d781\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-mapsparameters\" href=\"#tab-139ac17b0c82db5d781\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-filter fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Maps parameters<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-mapsparameters\" id=\"tab-139ac17b0c82db5d781\">\n<h4><span style=\"color: #008080;\">Check the\u00a0<img decoding=\"async\" class=\" wp-image-760 alignnone\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.18.49-AM.png\" alt=\"\" width=\"145\" height=\"34\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.18.49-AM-200x47.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.18.49-AM.png 264w\" sizes=\"(max-width: 145px) 100vw, 145px\" \/> button<\/span><\/h4>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:1px;border-bottom-width:1px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p>Details:\u00a0loading the ligand initialized the list of maps to be computed to the list of <em>AutoDock<\/em> atom types found in the ligand, in this case: \u201cA C Cl HD N NA OA\u201d.\u00a0Computing maps for all atoms types will use a little more disk space but the resulting target file can be used for any ligand and is recommended.<\/p>\n<p><img decoding=\"async\" class=\" wp-image-758 aligncenter\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.16.55-AM.png\" alt=\"\" width=\"513\" height=\"315\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.16.55-AM-200x123.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.16.55-AM-300x184.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.16.55-AM-400x246.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.16.55-AM-600x369.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.16.55-AM-768x472.png 768w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.16.55-AM.png 784w\" sizes=\"(max-width: 513px) 100vw, 513px\" \/><\/p>\n<p>The \u201cedit \u2026\u201d button will display an interface for manually specifying the list of atoms types for which affinity maps are requested.<\/p>\n<p><strong>Water maps<\/strong>: water maps\u00a0 are calculated automatically and stored in the target file. These maps allow to perform <a href=\"https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/jm2005145\">hydrated docking<\/a>. the parameter for these maps can be set in the &#8220;water map setting&#8221; pull down.<\/p>\n<p><img decoding=\"async\" class=\" wp-image-759 aligncenter\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.17.06-AM.png\" alt=\"\" width=\"162\" height=\"110\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.17.06-AM-200x136.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.17.06-AM-300x204.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.17.06-AM.png 350w\" sizes=\"(max-width: 162px) 100vw, 162px\" \/><\/p>\n<p><strong>Gradients<\/strong>: By default, an affinity gradient will be created for the region of the grid covered by the receptor (except for the electrostatic and desolvation maps). If your CPU has multiple cores and OpenMP is detected, the calculation will execute in parallel and the number of available threads appears in the GUI title bar. Gradients are not required, however, they are recommended as they have been shown to\u00a0help docking find solutions more efficiently. The addition of gradients requires the definition of the <em>interior<\/em> and <em>exterior<\/em> of the receptor. By default, the largest cluster of negative values (favorable affinities) is used to define the exterior of the receptor. In this case, everything else, including receptor cavities large enough to hold solvent but not open to the solvent, will be considered the interior and will be overwritten with the gradient. To prevent the gradient generation on grid points of internal cavities, the user can specify a cluster size above which clusters of points with negative values are considered to be outside the receptor and therefore preserve their original affinity values.<\/p>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-b6d089c44ce9385d9ec\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-generatemaps\" href=\"#tab-b6d089c44ce9385d9ec\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-calculator fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>Generate maps<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-generatemaps\" id=\"tab-b6d089c44ce9385d9ec\">\n<h4><span style=\"color: #008080;\">Click on the \u201cgenerate target file\u2026\u201d button\u00a0<\/span><\/h4>\n<div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:1px;border-bottom-width:1px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div>\n<p>Details: the program asks for the name and location for the target file that will be generated. It will then compute the affinity maps and store them in the target file along with meta data.<\/p>\n<p><img decoding=\"async\" class=\"aligncenter wp-image-763\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.28.11-AM.png\" alt=\"\" width=\"353\" height=\"212\" \/><\/p>\n<p>The generated target file describes the binding pocket targeted for docking on the receptor. It stores the PDBQT file of the receptor used to compute the affinity maps, the grid parameter file used to run <em>AutoGrid,<\/em> as well as files generated by the <em>AutoGrid<\/em>\u00a0run (<em>i.e.<\/em> affinity maps, <em>AutoGrid<\/em> log file, etc.). In addition, it stores meta-data about the generation of the target file such as: the time and computer architecture on which the maps were computed, the docking-box parameters, the grid parameter file, the versions of <em>AGFR<\/em> and\u00a0<em>AutoSite<\/em>, the water map parameters, etc.<\/p>\n<p>It is recommended to use names that are descriptive. In this case we use <em>NativeCDK2BindingSite_rigid<\/em> as we defined the docking box using the native ligand and we did not specify any flexible receptor side chains.<\/p>\n<p>Pressing the \u201cSave\u201d button will start the calculation in a separate thread, leaving the graphical user interface responsive. The progress bar below the button will indicate the level of completion of the calculation.<\/p>\n<p><img decoding=\"async\" class=\" wp-image-764 aligncenter\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.33.22-AM.png\" alt=\"\" width=\"323\" height=\"71\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.33.22-AM-200x44.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.33.22-AM-300x66.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.33.22-AM-400x88.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.33.22-AM-600x132.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.33.22-AM-768x169.png 768w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-15-at-11.33.22-AM.png 792w\" sizes=\"(max-width: 323px) 100vw, 323px\" \/><\/p>\n<p>NOTE: a target file can be inspected from the command line using the <em>about<\/em> command (<em>AutoDockSuite-1.0\/bin\/about<\/em>)<\/p>\n<\/div><div class=\"nav fusion-mobile-tab-nav\"><ul class=\"nav-tabs\" role=\"tablist\"><li role=\"presentation\"><a class=\"tab-link\" data-toggle=\"tab\" role=\"tab\" aria-controls=\"tab-8b65585dfa49888edc6\" aria-selected=\"false\" tabindex=\"-1\" id=\"mobile-fusion-tab-cmdline\" href=\"#tab-8b65585dfa49888edc6\"><h4 class=\"fusion-tab-heading\"><i class=\"fontawesome-icon fa-calculator fas\" aria-hidden=\"true\" style=\"font-size:13px;\"><\/i>cmd line<\/h4><\/a><\/li><\/ul><\/div><div class=\"tab-pane fade fusion-clearfix\" role=\"tabpanel\" tabindex=\"0\" aria-labelledby=\"fusion-tab-cmdline\" id=\"tab-8b65585dfa49888edc6\">\n<pre><span style=\"color: #008000;\"><style type=\"text\/css\" scopped=\"scopped\">.fusion-syntax-highlighter-1 > .CodeMirror, .fusion-syntax-highlighter-1 > .CodeMirror .CodeMirror-gutters {background-color:var(--awb-color1);}<\/style><div class=\"fusion-syntax-highlighter-container fusion-syntax-highlighter-1 fusion-syntax-highlighter-theme-light\" style=\"opacity:0;margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;font-size:14px;border-width:1px;border-style:solid;border-color:var(--awb-color3);\"><div class=\"syntax-highlighter-copy-code\"><span class=\"syntax-highlighter-copy-code-title\" data-id=\"fusion_syntax_highlighter_1\" style=\"font-size:14px;\">Copy to Clipboard<\/span><\/div><label for=\"fusion_syntax_highlighter_1\" class=\"screen-reader-text\">Syntax Highlighter<\/label><textarea class=\"fusion-syntax-highlighter-textarea\" id=\"fusion_syntax_highlighter_1\" data-readOnly=\"nocursor\" data-lineNumbers=\"\" data-lineWrapping=\"\" data-theme=\"default\" data-mode=\"text\/x-sh\">agfr -r data\/4EK3_rec.pdbqt -l data\/4EK4_lig.pdbqt -as<\/textarea><\/div>\r\n<\/span><div class=\"fusion-sep-clear\"><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><div class=\"fusion-separator-border sep-double sep-solid\" style=\"--awb-height:20px;--awb-amount:20px;border-color:#e0dede;border-top-width:2px;border-bottom-width:2px;\"><\/div><\/div><div class=\"fusion-sep-clear\"><\/div><\/pre>\n<p>Details: The target file produced by this tutorial can be generated from the command line using this command.<\/p>\n<p>-r :\u00a0\u00a0\u00a0 specifies the receptor file<\/p>\n<p>-l :\u00a0\u00a0\u00a0 specifies the ligand file<\/p>\n<p>-as :\u00a0 tells <em>AGFR<\/em> to use the original AutoSite algorithm rather than version 1.1<\/p>\n<p>NOTE: By default, when a ligand is specified on the command line, <em>AGFR<\/em> will use this ligand to position and size the box. For more command line options use the -h\/&#8211;help command line argument with the <em>agfr<\/em> command.<\/p>\n<p>The command provided above produces the following output:<\/p>\n<p><img decoding=\"async\" class=\"alignleft size-full wp-image-828\" src=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM.png\" alt=\"\" width=\"1035\" height=\"834\" srcset=\"https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-177x142.png 177w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-200x161.png 200w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-300x242.png 300w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-400x322.png 400w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-600x483.png 600w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-768x619.png 768w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-800x645.png 800w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM-1024x825.png 1024w, https:\/\/ccsb.scripps.edu\/agfr\/wp-content\/uploads\/sites\/33\/2019\/04\/Screen-Shot-2019-04-17-at-9.21.21-AM.png 1035w\" sizes=\"(max-width: 1035px) 100vw, 1035px\" \/><\/p>\n<\/div><\/div><\/div><div class=\"fusion-clearfix\"><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>This page was last updated on May 15th, 2019 at  [&#8230;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-736","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/pages\/736","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/comments?post=736"}],"version-history":[{"count":22,"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/pages\/736\/revisions"}],"predecessor-version":[{"id":902,"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/pages\/736\/revisions\/902"}],"wp:attachment":[{"href":"https:\/\/ccsb.scripps.edu\/agfr\/wp-json\/wp\/v2\/media?parent=736"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}