These illustrations show a model of an entire Mycoplasma genitalium cell. The model was created by Martina Maritan and Ludovic Autin at Scripps Research in the laboratory of Arthur J. Olson and David S. Goodsell. The protein concentration, length of DNA and mRNA, location of RNA polymerase and other DNA-binding proteins in the nucleoid, and location of ribosomes translating mRNA have been derived from computational simulations of a wild-type M. genitalium cell by Jonathan Karr and Markus Covert.
This snapshot represents a Mycoplasma cell at the beginning of its life cycle. Cell shape has been approximated to a sphere with a radius of ~145nm. Each protein is represented by a 3D structure coming from either homology modeling, a previous model of mycoplasma cytoplasm, experimental data, or homologues from the Protein Data Bank. The nucleoid structure was modeled with LatticeNucleoid. Small molecules, ions, and water are not shown in the illustrations, and would fill the spaces between the macromolecules. Different clipping planes and coloring schemes have been applied to the model to highlight specific areas/elements of the cell.
Detailed descriptions of the data curation, model building, and visualization methods can be found in the Journal of Molecular Graphics article: ‘Building Structural Models of a Whole Mycoplasma Cell’, https://doi.org/10.1016/j.jmb.2021.167351
These illustrations are freely available for use under a CC-BY-4.0 license. Right-click on the image to download the full-size image.
When you use these illustrations, please include the attribution: “Image by Martina Maritan, Scripps Research.”